Once again Andy Murray disappointed his fans, and this time it was sensational.
The 23-year-old Australian edged out the world number one in straight sets, putting the local crowd in disbelief 7-6 (4) 6-2.
Thompson had lost to Frenchman Jeremy Chardy on Sunday in the second round of qualifying but was handed a lifeline after Bedene pulled out with a wrist injury.
Aljaz Bedene, another British player, made a decision to quit and the Australian filled in the empty spot.
"I've looked up to him and that's definitely the biggest win of my career", a decidedly-chuffed Thompson told the BBC.
The Scot, who donated his £73,000 winnings from the same tournament in 2013 to charity, is aiming to win the event for a sixth time as he continues his preparation for Wimbledon.
The first set was inspiring, and neither of the players lost their serve.
While Thompson was delighted with his own game, he did concede Murray was not at his best.
And then, the hurricane occurred. Yeah, but most of those points landed first serves so it makes it tough on grass.
Twelve months later Murray was involved in the "Rally for Bally" at the Queen's Club, which raised more than £50,000 in memory of the former British No 1, Elena Baltacha, who died of liver cancer at the age of just 30. "If I play like that, I certainly won't win Wimbledon but I can play better than that". "The first set there was not much in it". Murray said he will consider playing in a grass-court exhibition event, either at Boodles or Hurlingham, to get some precious match time. It seems that the totally opposite thing happened. "Most of that comes down to confidence in matches, really".
"A big blow, for sure".
Andy Murray prepares for the Aegon Championships at Queens Club.
"But guys in the past have gone in to Wimbledon having not won lots of matches". His throne will be in danger if he continues to play like this, even after Wimbledon.
The Australian sealed victory with an ace down the middle as he became the first player since Roger Federer to hold serve for an entire match against Murray.