The IBF and WBA heavyweight champion was hoping to face Klitschko in a repeat of their thrilling April clash on November 11 in Las Vegas.
However, the 31-year-old may have to side with opportunity on the undercard of the rematch of Anthony Joshua and Vladimir Klitschko, which is provisionally pencilled in for November in Las Vegas. Many pundits had signalled an end was near soon, and that Klitschko would be better remembered after retirement and that his status as an all-time great fighter would be better recognized.
Klitschko last fought on April 29 in sold-out fight at Wembley Stadium in London - his 29th heavyweight title fight appearance. That began an 11-year reign at the top of the heavyweight division, the second-longest in history, including the third most successful consecutive defenses in history with 18. "I'm very thankful for this".
During a career spanning 69 fights - of which 64 were wins - Klitschko mastered four languages and earned a PhD in sports science from the University of Kiev.
FILE - In this March 20, 2010 file photo, Ukrainian champion Wladimir Klitschko reacts after winning the IBF, WBO and IBO heavyweight boxing title match over Eddie Chambers of the US, in Duesseldorf, western Germany.
Built an undefeated record of 24-0 with 22 knockouts before suffering his first loss to Ross Puritty in Klitschko's only professional bout in Ukraine.
Image Anthony Joshua defeated Wladimir Klitschko at Wembley Stadium in April
"Boxing is tough and it is the game for the young, youth and energetic, so I know that at 41, Klitschko retired at the right time, and should not be perceived as otherwise".
That was ended by another Briton, Tyson Fury, in Dusseldorf at the cost of his WBA, IBF and WBO titles.
Showing the global approach that helped make him such a marketable athlete, Klitschko released his farewell video Thursday in three languages - English, German and Russian. There was always the worry with Klitschko's age that we could announce the fight and he could get injured in camp, various bits and pieces like that.
There has already been speculation in British media that unification fight could happen early next year - providing Parker retains his belt in his second defence against Britain's Hughie Fuiry in Manchester on September 23 and Joshua still ahs his two titles.
The Joshua fight was validation Klitschko felt he needed to give to the fans, though he didn't win.
Eddie Hearn has confirmed Wladimir Klitschko's retirement means Anthony Joshua must fight the mandatory challenger to his IBF title.