The 60,000 plus spectators jeered Gatlin whilst chanting bronze medal victor Bolt's name as if he were the champion.
Many countries also pay their athletes for competing and winning at the Olympics, and Jamaica is no exception.
The world of athletics would have wanted another passing of the baton at a time when its biggest star, the CV clean and without the slightest suspicion, has made a decision to hang up the spikes.
Usain earned his "Lightning Bolt" nickname as a young boy in Jamaica, but it was his speed at a May 2008 meet in New York City when the press took note.
Instead of focusing on the 400m, Bolt wanted his second race to be the 100m. In a separate report by BBC News, he holds the world record in 100m race with 9.58 seconds.
He continued: "So you play all that with the slight complication of the technical issues and you end up the performance that Usain really gave us which was, he actually just drove out the blocks and ran and chased instead of him doing his lovely normal fluid action and just take it in time and move himself through".
It's a disappointing ending expected to be the final race for the greatest sprinter ever, which - nooooooooooo don't leave us Usain.
He is untouchable in the Olympics and the World Championship.
Danny Talbot, Adam Gemili, Nethaneel Mitchell-Blake and CJ Ujah ran a collective time of 37.47 seconds to beat favourites U.S. to first place and claim gold - their first since 2004.
Seeing Bolt beaten in his retirement run was a shock, but it also should have been a moment to be celebrated. They were the second quickest qualifiers behind the United States and go into the Saturday evening final with high hopes of a medal.
Usain Bolt is now retired.
Bolt's athleticism was way ahead of its time.
Asked if he expects the London crowd to boo Gatlin again during the 4x100m relay race on August 12, Coe simply said, I think the crowd would want to watch fantastic athletics.
But it's more than his talent that deserves a thumbs up.
But for the grand entrance of Bolt into the scene, athletics was nearly being ruined by the several doping scandals that had marred the image of the sport.
In 2006, he was banned for eight years, later reduced to four on appeal, after testing positive for the banned steroid testosterone.
It was never like that when Bolt was doing his thing.
No one is flawless, it is argued, and although Gatlin has received two suspensions for failed drugs tests, people point to the circumstances around his first ban, and giving him another chance after his second.
If you read about Bolt's rise to prominence from his early days playing in the streets and primary school playground in Sherwood Content, you would learn that he has always stood out among his peers as physically superior.
"Back home in Trowbridge my mum has a pic of it". However, and until sport universally sorts itself out, does it make it right to boo one person whilst cheering others who have committed similar crimes?
But I maintain that the only difference between Bolt and Gatlin in this respect is that Bolt was smarter, luckier, or better protected.